Trichomonas vaginalis

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Scientific classification
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Between 1934 and 1939, Trichomonas vaginalis was identified and classified by Procaccini, an Italian scientist, in Ethiopia. Little information is known of the discovery of Trichomonas vaginalis. Trichomonas vaginalis, a flagellated protozoan, is the causative agent of trichomoniasis belongs to the kingdom Archezoa. The members of this group, E. histolytica and G. lamblia, are parasitic protozoas that adhere to the host lumen, are fermenters (anaerobic respiration), and lack mitochondria. Instead of mitochondria they have a hydrogenosome that generate molecular hydrogen and produce ATP, among its many functions. All the members of Archezoa also have eight flagella and 2 nuclei. Five flagella are near the cytosome, four of which extend outside the cell together. The fifth flagellum’s functionality is the least understood; it wraps along the surface of the organism. Opposite the four flagella that extends outward, lies the barb-like axostyle. The axostyle forms a complex of cross-linked microtubules. The axostyle may be used for attachment to host lumen and can cause the tissue damage noted in trichomoniasis infections. The flagella and axostyle are distinguishing features of T. vaginalis. T. vaginalis is a sexually transmitted infection that can cause sexually transmitted disease in humans; mostly affects the female urinal tract. It is important to isolate the microorganism in the urine by examining morphology and motility. According to the WHO, there are approximately 180 million cases of T. vaginalis worldwide and according to the CDC, 7.4 million new cases occurs in the United States of America.This creates an important health concern.

T. vaginalis is an anaerobic protozoan, which uses a hydrogenosome to break down pyruvate into carbon dioxide. In other words, the hydrogenosome functions as a respiratory organelle. Glycolysis takes place with in the hydrogenosome by converting glucose to glycerol and succinate in the cytoplasm. This is followed by converting pyruvate and malate to hydrogen and acetate. It is thought that the hydrogenosome came before the mitochondrion on the phylogenetic tree. Interestingly, the adhesins AP65, AP51, and AP33 mediating binding to vaginal epithelial cells share identity to enzymes of the hydrogenosome organelle and further testing has shown how metabolism is linked to host adherence.

T. vaginalis has a variety of shapes ranging from ellipsoidal, ovoidal, and spherical. Giant forms, 30-50 mm in diameter, have been reported. Changes in cell shape are due to changes in pH, temperature, oxygen tension, and ionic strength. According to Fine structure of Trichomonas vaginalis using electron tomography, T. vaginalis has two different types of flagella; an anterior flagella and a recurrent flagella. It also has different types of cytoskeleton including parabasal filaments, pelta axostyle, and the costa. The costa, found only in trichomonads with an undulating membrane, is assumed to provide mechanical support and the axosyle is a bundle of microtubules.

Trichomonads infection leads to abnormal vaginal ecology, which leads to the contraction of a sexually transmitted disease. T. vaginalis is used as a measure to detect HIV. In the study, Dependence on p38 MAPK signaling in the up-regulation of TLR2, TLR4 and TLR9 gene expression in Trichomonas vaginalis-treated HeLa cells, epithelial cells infected with T. vaginalis show an correlation with HIV entry. The T. vaginalis manipulates the p38 Mitogen Activated Protein kinase pathway to increase cytokine expression. P38MAPK is linked to the programmed cell death (PCD) in response to stressors, such as ultraviolet light, osmotic stress, trophic factors, and growth factors. This causes PCD in epithelial cells. Programmed cell death of epithelial cells facilitates the entry of HIV and other STD’s. This study also showed how p38 inhibition decreased tumor necrosis factor alpha, which is involved in T. vaginalis proliferation and differentiation. P38 inhibition has been studied for treatments for cancer and inflammation. T. vaginalis causes inflammation of the vagina and vulva in females.

Pathology

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Application to Biotechnology

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Current Research

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