Mitochondrion: Difference between revisions
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==General characteristics== | |||
Usually seen as inflexible cylinders of roughly 0.5–1 μm, mitochondria resemble bacteria. Mitochondria are actual very mobile and frequently change shape. They may even fuse with other mitochondria and then separate. They are often associated with microtubules in the cytoplasm which determine mitochondrial orientation and distribution. In some cells they form long moving chains and in other types of cells they remain fixed in a position providing ATP directly to a site of high ATP consumption such as the adjacent myofibrils of cardiac muscle or the flagellum of sperm.<ref name=AlbertTheMito/> | |||
Mitochondria were first seen in a light microscope in the nineteenth century but were not really understood until procedures for isolating intact samples were developed in 1948. Mitochondria are often retrieved from purified liver cells which contain from 1000 to 2000 mitochondria each, occupying about one-fifth of the cell volume.<ref name=AlbertTheMito/> | |||
==Substructure== | ==Substructure== | ||
The two membranes are separated by space. The space enclosed within the inner memberane is the matrix, a moderately dense region with strands of DNA, ribosomes, or small granules. Using these tools, the mitochondria code for a portion of their own proteins. <ref name=UTMedMitoStrx/> | There are two membranes and two internal compartments or spaces. The two membranes are separated by the inter-membrane space. The space enclosed within the inner memberane is the matrix, a moderately dense region with strands of DNA, ribosomes, or small granules. Using these tools, the mitochondria code for a portion of their own proteins. <ref name=UTMedMitoStrx/> | ||
===Inner membrane=== | ===Inner membrane=== | ||
Revision as of 18:59, 13 January 2008
Template:TOC-right Mitochondria (singular mitochondrion) are the source of energy production within a cell. They are semiautonomous and self producing, residing in the cytoplasm of eukaryotic cells. Converting cellular energy metabolites in the Kreb’s Cycle, through the process of oxidative phosphorylation they produce adenosine triphosphate (ATP) which is used to power other processes in the cell.[1][2][3][4]
Their function is essential to efficient energy production. Without them eukaryotic cells would be dependent on anaerobic glycolysis for their ATP. Glycolysis releases very little free energy but in the mitochondria the metabolism of sugars is much more efficient and provides 15 times more ATP than is produced through glycolysis.[5]
Mitochondria take up a large portion of the cytoplasmic volume of eukaryotic cells. They are rod shaped[6] organelles [7] with an inner and an outer membrane. The outer membrane limits the organelle. The inner membrane folds in on itself forming the cristae mitochondriales, giving the appearance of partitions and chambers within the organelle in cross section.[8] The cristae number and shape vary according to the type of tissue and organism. Cristae serve to increase the surface area of the inner membrane.[2].
Mitochondria contain their own genome which is separate and distinct from the genome of the cell.[2] Theoretically, mitochondria may have been separate unicellular organisms at one time and were subsumed in a symbiotic relationship into eukaryotic cells at some point in the evolutionary process.[9]
General characteristics
Usually seen as inflexible cylinders of roughly 0.5–1 μm, mitochondria resemble bacteria. Mitochondria are actual very mobile and frequently change shape. They may even fuse with other mitochondria and then separate. They are often associated with microtubules in the cytoplasm which determine mitochondrial orientation and distribution. In some cells they form long moving chains and in other types of cells they remain fixed in a position providing ATP directly to a site of high ATP consumption such as the adjacent myofibrils of cardiac muscle or the flagellum of sperm.[5]
Mitochondria were first seen in a light microscope in the nineteenth century but were not really understood until procedures for isolating intact samples were developed in 1948. Mitochondria are often retrieved from purified liver cells which contain from 1000 to 2000 mitochondria each, occupying about one-fifth of the cell volume.[5]
Substructure
There are two membranes and two internal compartments or spaces. The two membranes are separated by the inter-membrane space. The space enclosed within the inner memberane is the matrix, a moderately dense region with strands of DNA, ribosomes, or small granules. Using these tools, the mitochondria code for a portion of their own proteins. [3]
Inner membrane
The inner membrane constitutes the framework for electron-transport processes that produce most of the cell's ATP.[9]
In prokaryotes, the plasma membrane is used to produce ATP. However, eukaryotic cells reserve this function for membranes in energy converting organells, mitochondria and plastids[10] and the plasma membrane is reserved for other transport processes. Mitochondria and plastids are morphologically notable for their extensive inner membranes. [9]
Outer membrane
The external membrane of the mitochondria contain the transport protein porin. Porin forms aqueous channels through the lipid bilayer of the mitochondria, resembling a permeable sieve through which molecules, including small proteins, of 5000 daltons or less may pass into the inter-membrane space.
The inner membrane, by comparison is impermeable to most molecules. In other words, the inter-membrane space is chemically like the cytosol while the internal matrix of the mitochondria contains a select set of molecules.[5]
Life cycle
Replication
In a process similar to replication in bacterial cells, when a mitochondrion reaches a certain size, they undergo fission, the furrowing of the inner and outermembrane which pinches them into two daughter mitochondria. Prior to fission they replicate their DNA.[11]
Death
Mitochondrion at the end of their life are disposed of through autophagy. Cellular endoplasmic reticulum are wrapped around the mitochondrion forming a vacuole. Golgi complex vesicles containing hydrolases then join with the autophagic vacuole which then degrades the contents of the vacuole, the mitochondrion.[11]
Notes
- ↑ [1] Munich Information Center for Protein Synthesis
- ↑ 2.0 2.1 2.2 What is a cell? National Center for Biotechnology Information, National Library of Medicine, National Insitutes of Health
- ↑ 3.0 3.1 Mitochondrial substructure Cell Biology Graduate Program, University of Texas Medical Branch
- ↑ ATP and oxidative phosphorylation reactions Jakubowski, Henry (2006) College of Saint Benedict, Saint John’s University
- ↑ 5.0 5.1 5.2 5.3 The mitochondrion Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P. (2002) Molecular Biology of the Cell. NY:Taylor & Francis Group, Garland Science
- ↑ Usually rod shaped. they may also be round.
- ↑ a word derived from Greek meaning Little organ
- ↑ Mitochondria: Architecture dictates function Cell Biology Graduate Program, University of Texas Medical Branch
- ↑ 9.0 9.1 9.2 Energy Conversion: Mitochondria and Chloroplasts Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P. (2002) Molecular Biology of the Cell. NY:Taylor & Francis Group, Garland Science
- ↑ notably chloroplasts—occuring only in plants
- ↑ 11.0 11.1 The mitochondrial life cycle Cell Biology Graduate Program, University of Texas Medical Branch