Legionella pneumophila

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Legionella pneumophila
Scientific classification
Kingdom: Bacteria Phylum: Proteobacteria Class: Gamma Proteobacteria Order: Legionellales Family: Legionellacease Genus: Legionella Species: L. pneumophila
Binomial name
Legionella pneumophila

Description and significance

Legionella pneumophila is a rod shaped, flagellated and a gram negative bacteria. L. pneumophila are aerobic and non-capsulated. It is known to be pathogenic to humans and leads to legionellosis or legionnaires’ disease. The name Legionnaires comes from an outbreak of this disease during a convention of American Legions in 1976. L. pneumophila is determined to be the dominant human pathogen in its genus of 41 different species . It was first found to be pathogenic in 1976 when a group of men were found to be infected. It spread to more than 200 individuals and led to 34 casualties. It was first isolated by inoculation of a guinea pig with the blood sample of an infected person in 1947.

Genome structure

There are three genomes from the species L. pneumophila that have been studied well. These are Legionella pneumophila str. Paris, Legionella pneumophila str. Lens and Legionella pneumophila ssp. Pneumophila str. Philadelphia 1. All three of these genomes have been completed recently between 2001 and 2004. As discussed later, one of the most essential functions in L. pneumophila’s pathogenic behavior is the escaping from the host cell after having multiplied. This use of type IV secretion has been identified and studied during the process of lysis. The gene related to type IV secretion has been found to be dot/icm gene complex.

Name	Genes	Base Pairs	Year
Legionella pneumophila str. Paris	3136	3,503,610	2004
Legionella pneumophila str. Lens	3001	3,345,687	2004
Legionella pneumophila ssp. Pneumophila str. Philadelphia 1	3002	3,397,754	2001

Cell structure and metabolism

L. pneumophila is a flagellated gram negative bacteria. It has a rod-like (bacillus) shape, and use aerobic respiration. They are unable to capsulate, sporulate or ferment. As for light, they do not have pigments and are unable to carry on autflouresce. Although it is a gram negative bacteria, it is still difficult to stain L. pneumophila due to certain lippolysaccharides in their cell membrane. A minimum of 35 antigens have already been identified from L. pneumophila. As for the size, it is approximately 2 µm in length and 0.3-0.9 µm in width. The shape changes, by increasing the length, when under poor nutritional conditions. The cell membrane contains a lipopolysaccharide which initiates the immune response of the host. This lipopolysaccharide has been extensively researched. The lipopolysaccharide in L. pneumophila has a significantly weaker interaction with CD14 receptor proteins of others cell, compared to the interaction strength of lipopolysaccharides found in other bacteria, such as enterobacterial. This weaker interaction reduces the endotoxic effects of L. pneumophila. The antigens located on the cell membrane are used to identify the different serogroups in the genus pneumophila.

Ecology

Schematic sources for L. pneumophila

In general L. pneumophila can be found in aquatic environments worldwide. L. pneumophila can be found in many large water systems, such as those used in buildings. It often ends up in the heating and cooling components of residential apartments. If such water systems are left unchecked, it can lead to outbreaks of Legionnaires’’ disease can occur. Furthermore studies have shown that L. pneumophila thrives in water tanks for two primary reasons. Firstly many water tanks show the presence of sediments, which acts as a nutrient source for L. pneumophila. Secondly many water tanks are kept at elevated temperature, which makes it easier for L. pneumophila to survive.

Pathology

L. pneumophila is known to infect protozoa’s mostly but sometimes these infections also occur in humans. L. pneumophila enters the human body through the lungs and attacks the immune cells. When invading macrophages in humans it behaves similarly to when it invades protozoan cells. When L. pneumophila enters a cell, it inhibits phagolysosome fusion, which disables the cells major defense mechanism. Then L. pneumophila multiplies within a membrane, which is known to behave similarly to endoplasmic reticulum. Finally when is it ready to release, it uses type IV secretion system to lysis the host cell. This causes an inflammatory response in humans, which worsens the situation because it provides more macrophages for L. pneumophila invasions. Infection by L. pneumophila in humans is called Legionnaires’ disease or Legionellosis. Once infected there are two possibilities: the person might develop pneumonia, which is the dangerous version of the disease or the person might develop Pontiac fever, which is usually nonfatal. Many cases of Legionnaires’ disease go unnoticed because infections of Legionnaires’ disease are often reported as respiratory infections and in some cases a treatment for respiratory infections can also treat the Legionnaires’ disease. People of all ages are susceptible to legionnaires’ disease but it is more fatal to people with chronic lung disease, smoking habits and a compromised immune system. Furthermore legionnaires’ disease can’t be passed on directly from person to person. In fact the only way for it to be passed on is through aerosolization or aspiration of water There are 41 different species in the genus Legionella and these are further divided into 64 serogroups. The serogroups 1, 4, and 6 are responsible for most human infections of Legionnaires’ disease. Serogroup 1 is determined to be the cause of 70-90% of such infections. Studies show approximately only 5-15% of Legionnaires’ disease cases are fatal. A recent case, in January 2009, of Legionnaires’ disease has occurred at a recently opened luxurious hotel in Dubai. Three individuals who had been residing at Westin Dubai Mina Seyahi Beach Resort reported severe illness after leaving the hotel. Furthermore one of the three individual, who was a famous Cricket Broadcaster in Britain, passed away after battling the disease for a short period. The other two individual continue to struggle with Legionnaires’’ disease. The hotel authorities have cooperated and allowed the testing for Legionella bacteria in the hotel; so far all results have returned negatively.

Macrolides (azithromycin) or fluoroquinolones (moxifloxacin) are two drugs that are commonly used for treating L. pneumophila in people.

Year	Male	Female	Total
1999	42	14	56
2000	125	29	154
2001	78	8	86
2002	139	29	167
2003	127	20	147
2004	151	9	160
2005	252	29	281
2006	452	66	518
2007	527	141	668
2008	529	157	686

Application to Biotechnology

Still researching on this topic… will be up by this weekend.

Current Research

Research I

L. pneumophila invasion of endothelial cells, wild type vs. mutant (dotI) with/without wortmannin

One study tests the ability of L. pneumophila’s ability to invade and reproduce in other types of cells in human body besides macrophages. In this case endothelial cells from human umbilical vein were used. There were two types of L. pneumophila bacteria were used; one was a strain called Lp01 and the other was a mutated form of L. pneumophila called dotI (HL051C). This mutant lacks the ability to inhibit phagolysosome fusion. Furthermore both type of L. pneumophila were tested with and without the presence of wortmannin, which inhibits PI-3 kinase. Wortmannin had previously demonstrated that the Lp01 strand (wild type) and dotI (mutant) use different pathways because wortmannin was successfully able to reduce invasions by dotI in macrophages, while having little effect on the wild type. For this to hold true, wortmannin should also demonstrate similar results when invading other types of cells. The results of this experiment show that overall invasion was nearly a hundred times less frequent in endothelial cells compared to macrophage cells. The primary reason being L. pneumophila find it easier to invade macrophages because they are specialized phagocytes. The second part of this study shows that when comparing the wild type and mutant, the mutant did show to reduced ability to invade endothelial cells but the difference was considered insignificant. As for the last part of this study, in the presence of wortmannin the mutant showed a significantly larger decline compared to the decline faced by the wild type.

Research II

Another recent study, April 18th 2009, revealed shocking data. Legionnaire’s disease, which was originally thought to be only possible by aerosols contaminations, is now showing signs of having spread through potting soil. Many cases have been reported to have occurred in Switzerland. In this study 46 samples of various commercial potting soils have been taken and tested for presence of legionnaire’s disease causing species. 21 of the 46 samples have confirmed the presence of Legionella spp. 9 of these samples showed the presence of L. pneumophila. Furthermore results from real-time PCR showed that these bacteria were present at high concentration. The potting soil available to the public in Switzerland could be a reservoir for L. pneumophila and other legionnaire’s disease causing bacteria, a high probability of an outbreak currently exist in Switzerland. This research shows that the potential of Legionnaire’s disease had been severely underestimated. Further research is being done to reaffirm the findings and to test other possible reservoir for Legionella spp.