Flow cytometry: Difference between revisions
imported>Howard C. Berkowitz mNo edit summary |
imported>Howard C. Berkowitz (Introduction generalized from laser/antigen and added Coulter Counter) |
||
| Line 1: | Line 1: | ||
{{subpages}} | {{subpages}} | ||
'''Flow cytometry (FC)''' is a laser-based fluorescence detection method used to characterize cell antigens on large numbers of [[blood cells|hematopoetic]] or [[lymphoid cell]]s in suspension typically derived from peripheral blood, bone marrow aspirate or lymph node tissue.<ref>{{citation | '''Flow cytometry (FC)''' is a method for characterizing the counts and size distribution of particles in fluid, such as blood cells. The original technique passed cells through pores in a membrane and measured the current produced; this was the Coulter Counter of 1953. Prior to this invention, blood cell counting was a laborious and variable manual procedure. <ref>{{citation | ||
| title = Wallace H. Coulter (1913-1998): Automated Blood Analysis | |||
| url = http://web.mit.edu/invent/iow/coulter.html | |||
| publisher = Inventor of the Week}}</ref> In current [[laboratory medicine]], it primarily is a laser-based fluorescence detection method used to characterize cell antigens on large numbers of [[blood cells|hematopoetic]] or [[lymphoid cell]]s in suspension typically derived from peripheral blood, bone marrow aspirate or lymph node tissue.<ref>{{citation | |||
| url = http://depts.washington.edu/labweb/Divisions/Hema/Flow.htm | | url = http://depts.washington.edu/labweb/Divisions/Hema/Flow.htm | ||
| publisher = Division of Laboratory Medicine, [[University of Washington]] | | publisher = Division of Laboratory Medicine, [[University of Washington]] | ||
| title = Flow Cytometry}}</ref> | | title = Flow Cytometry}}</ref> | ||
Laser-based flow cytometry instruments use a thin laminar flow of fluid to direct thousands of cells from a small diameter nozzle through a thin beam of laser light of a certain wavelength. The cells are categorized by size forward light scatter, cellular complexity or granularity side scatter and by antigen density fluorescence intensity for each 'CD' clusters of differentiation marker. The antigen distribution for thousands of cells is printed in graphical histograms which a pathologist interprets individually and collectively to determine the phenotype of dominant cell populations in [[leukemia]]s and [[lymphoma]]s<ref>{{citation | |||
| journal = Journal of Immunological Methods | | journal = Journal of Immunological Methods | ||
| volume=171 |issue=1| date = 2 May 1994| pages=131-137 | | volume=171 |issue=1| date = 2 May 1994| pages=131-137 | ||
Revision as of 11:01, 14 January 2011
Flow cytometry (FC) is a method for characterizing the counts and size distribution of particles in fluid, such as blood cells. The original technique passed cells through pores in a membrane and measured the current produced; this was the Coulter Counter of 1953. Prior to this invention, blood cell counting was a laborious and variable manual procedure. [1] In current laboratory medicine, it primarily is a laser-based fluorescence detection method used to characterize cell antigens on large numbers of hematopoetic or lymphoid cells in suspension typically derived from peripheral blood, bone marrow aspirate or lymph node tissue.[2]
Laser-based flow cytometry instruments use a thin laminar flow of fluid to direct thousands of cells from a small diameter nozzle through a thin beam of laser light of a certain wavelength. The cells are categorized by size forward light scatter, cellular complexity or granularity side scatter and by antigen density fluorescence intensity for each 'CD' clusters of differentiation marker. The antigen distribution for thousands of cells is printed in graphical histograms which a pathologist interprets individually and collectively to determine the phenotype of dominant cell populations in leukemias and lymphomas[3] of various types.
References
- ↑ Wallace H. Coulter (1913-1998): Automated Blood Analysis, Inventor of the Week
- ↑ Flow Cytometry, Division of Laboratory Medicine, University of Washington
- ↑ A.Bruce Lyons and Christopher R. Parisha (2 May 1994), "(Abstract) Determination of lymphocyte division by flow cytometry", Journal of Immunological Methods 171 (1): 131-137, DOI:10.1016/0022-1759(94)90236-4