Induced pluripotent stem cells

From Citizendium
Revision as of 17:23, 26 December 2007 by imported>Ro Thorpe
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search
This article is developing and not approved.
 Main Article
 Discussion
Related Articles  [?]
Bibliography  [?]
External Links  [?]
Citable Version  [?]
 
https://en.citizendium.org/wiki/index.php?title=Induced_pluripotent_stem_cells&printable=yes
This editable Main Article is under development and subject to a disclaimer.

Introduction

Induced pluripotent stem cells (iPS cells) are a class of stem cells that are artificially derived from differentiated embryonic or adult tissues. Therapeutically, iPS cells hold the potential to be used to treat a patient with their own cells (i.e. bone marrow transplant, spinal cord injury repair).

Multiple strategies to generate iPS cells are being developed.

  • Somatic cell nuclear transfer
  • Embryonic stem cell fusion
  • Nuclear reprogramming

Nuclear reprogramming

Based on previous studies, one research group set out to determine which genes are required to be expressed in order to generate/maintain embryonic stem cells.1 In a series of experiments it was determined that 4 factors are required to cause mouse fibroblasts to "dedifferentiate" into a stem-cell like state.

  • Sox2
  • Klf4
  • Oct3/4
  • c-myc

References

1. Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006 Aug 25;126(4):663-76. Epub 2006 Aug 10. PMID: 16904174 [PubMed - indexed for MEDLINE]

Retrieved from "https://citizendium.org/wiki/index.php?title=Induced_pluripotent_stem_cells&oldid=21151"