Yersinia pestis
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| Yersinia pestis |
Description and significance
Yersinia pestis is a nonmotile, non–spore-forming, pleomorphic, gram-negative, facultative anaerobic bipolar-staining bacillus bacterium belonging to the family Enterobacteriaceae. The bacteria elaborate a lipopolysaccharide endotoxin, coagulase, and a fibrinolysin, which are the principal factors in the pathogenesis of this disease. Yersinia pestis was discovered in 1894 by Swiss/French physician and bacteriologist from the Pasteur Institute, Alexandre Yersin. It was isolated during an epidemic of the plague in Hong-Kong and It was Yersin who actually linked the plague with Yersinia pestis. Originally named Pasteurella pestis, the microbe was renamed in 1967 after its founder.
Genome structure
Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Does it have any plasmids? Are they important to the organism's lifestyle?
The genetic material of Yersinia pestis is skein of circular DNA that is localized as the nucleoid, which lacks a nuclear membrane. The genome structure has been decoded for two of the three sub-species of Yersinia pestis, the KIM strain and the CO92 strain. The chromosome of the KIM strain contains 4,600,755 base pairs and the chromosome of the CO92 strain has 4,653,728 base pairs. There are 4,012 protein-coding genes, including 149 pseudogenes. The genome is rich in insertion sequences and displays anomalies in GC base-composition bias, which indicates frequent intragenomic recombination. Many genes seem to have been acquired from other bacteria and viruses, which suggests that Yersinia pestis is a pathogen that has undergone a large-scale genetic evolution. Yersinia pestis is also the host to the plasimds pCD1 (or pYV), pPCP1 (or pPst), and pMt1 (or pFra) which along with a pathogenicity island called HPI encode the proteins that cause the infamous pathogenicity of the bacteria. These virulence factors are essential for the invasion of the bacteria into the host, and the injection of its proteins into the cell. The pCD1 plasmid contains 70,300 bases with 4-8 copies per cell, pPCP1 has 9,600 bases with 100-200 copies per cell, and pMT1 has 96,200 bases with 1-2 copies per cell. The pMt1 plasmis encodes the F1 polysaccharide that forms the lumpy surface feature of the bacteria. This means that the chromosome is about 48 times the size of the biggest plasmid.
Cell structure and metabolism
Describe any interesting features and/or cell structures; how it gains energy; what important molecules it produces.
Yersinia pestis contains a cell wall that consists of an outer membrane that compromises an inner phospholipid layer and an outer lipopolysaccharide layer. There is also a middle peptidoglycan layer that lies exterior to the plasma membrane. The bacteria's cell wall is quite unlike the typical gram-negative enterobacterial cell wall as it lacks O-side chains due to a disrupted O-antigen gene cluster.
Under some circumstances, Yersinis pestis may be sorrounded by a loose capsule and is covereld by a slime envelope that is heat labile. The cytoplasm of the bacteris is filled with ribosomes are linked into polysomes. There is also a hypothesis that type III secretion needles may be demonstrated in Yersinia pestis at 37°C and that their formation is triggered when it comes in contact with other cells. Such needles have been demonstrated in its closely related species Yersinia enterocolitica.
Ecology
Describe any interactions with other organisms (included eukaryotes), contributions to the environment, effect on environment, etc.
Yersinia pestis is a gram negative bacteria that is a facultative anaerobe. During an outbreak the bacteria can survive for long periods of time in cool, moist areas such as the soil of rodent holes. It is easily destroyed by sunlight and drying, but even so when released into the air it may survive for up to one hour. The optimum temperature for growth of the bacteria is about 26° - 37°C. Between outbreaks the bacteria is believed to circulate within populations of several rodent species without causing excessive death. Such groups of infected animals serve as silent, long-term carriers of the infection.
The plague caused by Yersinia pestis is a zoonotic disease primarily affecting rodents. Humans do not play a role in the long-term survival of the bacterium. Fleas are the vectors of transmission in rodents as well as from rodents to humans. The most common method of transmission of the bacteria to humans is through the bite of an infected flea. After the fleas feed with a blood meal, they are believed to regurgitate bacteria back into uninfected animals.
Yersinia pestis contains enzootic and epizootic transmission cycles involving rodents and their fleas. Risk for plague in humans is greatest during the epizootic cycle because the mortality rate for rats is high, causing the fleas to seek alternative hosts, including humans. The plague caused by Yersinia pestis can be seperated into the Urban and Sylvatic cycles. In the Urban cycle the flea hosts are compromised of domestic rodents, while in the Sylvatic cycle the hosts are wild rodent populations.
Pathology
How does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.
Yersinia pestis is transmitted to people that have been bitten by infected fleas that are carried on rodents, most commonly which are rats, field mice, squirrel prairie dogs, rabbits and even animals such as cats and camels. The most common vector is the is the rat flea Xenopsylla cheopis, although ticks and human lice have been identified as possible vectors. Humans are accidental hosts in the natural cycle of this disease.
When the flea ingests blood that is infected with yersinia pestis, it produces a lipopolysaccharide endotoxin, coagulase, which causes the blood to clot and the bacteria multiply to the thousands. While the bacteria grows in the flea it loses it's capsule layer and while most of the organisms are phagocytosed a few are taken up by tissue macrophages, which are unable to kill the bacteria. The bactteria kill the macrophage and migrate to the lymph nodes where they are phagocytosed by the polymorphonuclear cells and mononuclear phagocytes, and multiply intracellularly. Afterwards with lysis the bacteria can invade distant organs and continue to multiply. The hem binding chromosomal locus is turned on which allows the aggregation of planar molecules which creates a block in the stomach causing the flea to be blood-frenzy. During these subsequent blood feedings, the bacteria are inoculated in a host’s. Once the infection has spread to the lungs, pneumonia is developed and through sneezing and coughing the bacteria spread into the air and contaminate new hosts.
The symptoms that the infected individuals have vary according to the type of plague that person is inflicted with. The plagues are all caused by Yersinia pestis but differ in how the person was infected, how the disease is transmitted and which organs the bacteria resides in. The most common plagues and their symptoms are as follows:
| Plague Type | Method of Transmission | Organ Inffected | Symptoms |
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| Bubonic | Flea bite or infection through skin | Lymph nodes | Buboes (swollen and tender lymph nodes), pustules, carbuncles, eschar, or papules at the site of the infected flea bite, lack of energy, weakness, abdominal pain, fever, headache, and chills |
| Septicemic | Flea bite or infection through skin | Blood stream | Nausea, vomiting, abdominal pain, diarrhea, hypotension, hepatosplenomegaly, delirium, seizures in children, shock, lack of energy, and fever |
| Pneumonic | Air | Lungs | Fever, chills, cough, chest pain, dyspnea, hemoptysis, purulent sputum, lethargy, hypotension, and shock |
| Pharyngeal | Air | Pharynx and throat | Soar throat, fever, pharyngeal erythema, painful and tender anterior cervical nodes |
Application to Biotechnology
Does this organism produce any useful compounds or enzymes? What are they and how are they used
Yersinia pestis has recently gained attention as a possible biological warfare agent and the Center for Disease Control and Prevention (CDC) has classified it as category A pathogen requiring preparation for a possible terrorist attack. It is an optimum choice for a bioweapon, as it is very easy to spread and is resistant to multiple drugs. Because of the delay between exposure to the bacteria and signs of illness, people could travel over a large area before becoming contagious and possibly infect others. It is also possible to be employed as a bioweapon, because the bacterium occurs in nature and could very easily be isolated and grown in a labrotory. If used as an aerosol attack it could cause cases of the pneumonic form of the plague from one to six days after infection. However manufacturing such a weapon requires further advanced knowledge and technology.
Current Research
Enter summaries of the most recent research here--at least three required
Plague research is being conducted by several government agencies in an effort to help in the diagnosis, treatment, and prevention caused by Yersinia pestis, as well as addressing the need to defend against possible bioterrorist-caused disease outbreaks. Specifically this research focuses on developing a vaccine against the pneumonic plague, developing antibiotics to prevent and treat infection, and most importantly studying and identifying genes and proteins in Yersinia pestis that infect the digestive tract of fleas and enable them to grow and function in humans. Specifically, scientists at the National Institute of Allergy and Infectious Diseases (NIAID) Rocky Mountain Laboratories (RML), found that three genes in Yersinia pestis change it from a harmless, long-term inhabitant in the flea’s mid gut to one that migrates and accumulates in its foregut. As a result of this change, the flea begins to starve, causing it to fanatically feed, during which it regurgitates the bacteria and hence transmits the plague. Although it was known for quite some time that the bacterium’s transmission is dependant on the fleas as hosts, there was little understanding about the molecular and genetic mechanisms by which this colonization occurs. They began experiments on three hemin storage genes (hms), which are abundant in red blood cells, and acts as the iron-containing part of the hemoglobin molecule that binds oxygen. To understand the role of these genes in the host Dr. Hinnebusch conducted experiments with Oriental rat fleas, in which he injected the normal Yersinia pestis bacteria and a mutant form which was missing the hms genes. After four weeks, the scientists found that only those fleas infected with the normal bacteria developed the foregut blockage, which was accompanied by a high rate of mortality. These results indicated that the hms genes are required for Yersinia pestis to cause the foregut blockage. Next, they highlighted both forms of Yersinia pestis with fluoresce green and after dissecting the host fleas noted how the mutant bacteria remained in the midgut while the normal bacteria had migrated to the foregut in many fleas which eventually, became packed with bacteria. Now other genes are being studied that may affect the bacteria’s ability to transmit infection and it was observed that the blockage that develops in the flea foregut breaks down at temperatures above 80 to 85 degrees Fahrenheit. Scientists are trying to determine why this occurs and if such temperature changes might suppress the products of hms or other genes.